The Structure, Format, Content, and Style of a Journal-Style
Scientific Paper
| Table of
Contents | FAQs |
Why a Scientific
Format?
The scientific format may seem confusing
for the beginning science writer due to its rigid structure which is
so different from writing in the humanities. One reason for using
this format is that it is a means of efficiently communicating
scientific findings to the broad community of scientists in a
uniform manner. Another reason, perhaps more important than the
first, is that this format allows the paper to be read at several
different levels. For example, many people skim Titles to find out what information is available on
a subject. Others may read only titles and Abstracts. Those wanting to go deeper may look at the
Tables and Figures in the Results, and so on. The take home point here is that
the scientific format helps to insure that at whatever level
a person reads your paper (beyond title skimming), they will
likely get the key results and conclusions.
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The Sections of the Paper
Most journal-style scientific papers are subdivided into the
following sections: Title, Authors
and Affiliation, Abstract, Introduction,
Methods, Results,
Discussion, Acknowledgments,
and Literature Cited, which parallel
the experimental process. This is the system we will use. This
website describes the style, content, and format associated with
each section.
The sections appear in a journal style paper in the following
prescribed order:
Section Headings:
Main Section Headings: Each main section of the paper
begins with a heading which should be capitalized,
centered at the beginning of the section, and double
spaced from the lines above and below. Do not
underline the section heading OR put a colon at the end.
Example of a main section heading:
INTRODUCTION
Subheadings: When your paper
reports on more than one experiment, use subheadings to help
organize the presentation. Subheadings should be capitalized
(first letter in each word), left justified, and
either bold italics OR underlined.
Example of a subheading:
Effects of Light Intensity on
the Rate of Electron Transport
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Title, Authors' Names, and Institutional
Affiliations
1. Function: Your paper should begin with a Title
that succinctly describes the contents of the paper. Use
descriptive words that you would associate strongly with the
content of your paper: the molecule studied, the organism used
or studied, the treatment, the location of a field site, the
response measured, etc. A majority of readers will find your
paper via electronic database searches and those search engines
key on words found in the title.
2. Title FAQs
3. Format:
- The title should be centered at the top of page 1
(DO NOT use a title page - it is a waste of paper for our purposes);
the title is NOT underlined or italicized.
- the authors' names (PI or primary author first) and
institutional affiliation are double-spaced from and centered
below the title. When more then two authors, the names are
separated by commas except for the last which is separated from
the previous name by the word "and".
For example:
Ducks Over-Winter in Colorado Barley Fields in
Response to
Increased Daily Mean Temperature
Ima Mallard, Ura Drake, and Woodruff Ducque
Department of Wildlife Biology, University of Colorado - Boulder
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The title is not a section, but it is necessary and important.
The title should be short and unambiguous, yet be an adequate
description of the work. A general rule-of-thumb is that the
title should contain the key words describing the work
presented. Remember that the title becomes the basis for most
on-line computer searches - if your title is insufficient, few
people will find or read your paper. For example, in a paper
reporting on an experiment involving dosing mice with the sex
hormone estrogen and watching for a certain kind of courtship
behavior, a poor title would be:
Mouse Behavior
Why? It is very general, and could be referring to any of
a number of mouse behaviors. A better title would be:
The Effects of Estrogen on the Nose-Twitch Courtship Behavior
in Mice
Why? Because the key words identify a specific behavior, a
modifying agent, and the experimental organism. If possible,
give the key result of the study in the title, as seen in the
first example. Similarly, the above title could be restated as:
Estrogen Stimulates Intensity of Nose-Twitch Courtship
Behavior in Mice
4. Strategy for
Writing Title.
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ABSTRACT
1. Function: An abstract
summarizes, in one paragraph (usually), the major aspects of
the entire paper in the following prescribed sequence:
- the question(s) you investigated (or
purpose), (from Introduction)
- state the purpose very clearly in the first or second sentence.
- the experimental design and methods
used, (from Methods)
- clearly express the basic design of the study.
- Name or briefly describe the basic methodology used without
going into excessive detail-be sure to indicate the key techniques
used.
- the major findings including key
quantitative results, or trends (from
Results)
- report those results which answer the questions you were
asking
- identify trends, relative change or differences, etc.
- a brief summary of your interpetations and
conclusions. (from Discussion)
- clearly state the implications of the answers your results
gave you.
Whereas the Title
can only make the simplest statement about the content of
your article, the Abstract allows you to elaborate more on each
major aspect of the paper. The length of your Abstract should
be kept to about 200-300 words maximum (a typical standard length
for journals.) Limit your statements concerning each segment
of the paper (i.e. purpose, methods, results, etc.) to two or
three sentences, if possible. The Abstract helps readers decide
whether they want to read the rest of the paper, or it may be
the only part they can obtain via electronic literature searches
or in published abstracts. Therefore, enough key information
(e.g., summary results, observations, trends, etc.) must be included
to make the Abstract useful to someone who may to reference your
work.
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How do you know when you
have enough information in your Abstract? A simple rule-of-thumb
is to imagine that you are another researcher doing an study
similar to the one you are reporting. If your Abstract was the
only part of the paper you could access, would you be happy with
the information presented there?
2. Style: The Abstract is ONLY text. Use the active voice
when possible, but much of it may require passive constructions.
Write your Abstract using concise, but complete, sentences, and
get to the point quickly. Use past tense. Maximum length
should be 200-300 words, usually in a single paragraph.
The Abstract SHOULD NOT contain:
- lengthy background information,
- references to other literature,
- elliptical (i.e., ending with ...) or incomplete sentences,
- abbreviations or terms that may be confusing to readers,
- any sort of illustration, figure, or table, or references
to them.
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3. Strategy:
Although it is the first section of your paper, the Abstract,
by definition, must be written last since it will summarize the
paper. To begin composing your Abstract, take whole sentences
or key phrases from each section and put them in a sequence which
summarizes the paper. Then set about revising or adding words
to make it all cohesive and clear. As you become more proficient
you will most likely compose the Abstract from scratch.
4. Check your work: Once you have the completed
abstract, check to make sure that the information in the abstract
completely agrees with what is written in the paper. Confirm
that all the information appearing the abstract actually
appears in the body of the paper.
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INTRODUCTION
1. Function: The function of the Introduction is to:
- Establish the context of the work being reported. This is
accomplished by discussing the relevant primary
research literature (with citations)
and summarizing our current understanding of the problem you
are investigating;
- State the purpose of the work
in the form of the hypothesis, question, or problem you investigated;
and,
- Briefly explain your rationale
and approach and, whenever possible, the possible outcomes your
study can reveal.
Quite literally, the Introduction must answer the questions,
"What was I studying? Why was it an important
question? What did we know about it before I did this
study? How will this study advance our knowledge?"
2. Style: Use the active voice
as much as possible. Some use of first person is okay, but do
not overdo it.
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3. Structure: The structure
of the Introduction can be thought of as an inverted triangle
- the broadest part at the top representing the most general
information and focusing down to the specific problem you studied.
Organize the information to present the more general aspects
of the topic early in the Introduction, then narrow toward the
more specific topical information that provides context, finally
arriving at your statement of purpose and rationale. A good way
to get on track is to sketch out the Introduction backwards;
start with the specific purpose and then decide what is the scientific
context in which you are asking the question(s) your study addresses.
Once the scientific context is decided, then you'll have a good
sense of what level and type of general information with which
the Introduction should begin.
Here is the information should flow in your Introduction:
- Begin your Introduction by clearly identifying the subject
area of interest. Do this by using key words from
your Title in the first few sentences of
the Introduction to get it focused directly on topic at the appropriate
level. This insures that you get to the primary subject matter
quickly without losing focus, or discussing information that
is too general. For example, in the mouse behavior paper, the
words hormones and behavior would likely appear
within the first one or two sentences of the Introduction.
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- Establish the context by providing a brief and
balanced review of the pertinent published literature that is
available on the subject. The key is to summarize (for the
reader) what we knew about the specific problem before
you did your experiments or studies. This is accomplished with
a general review of the primary research literature (with
citations)
but should not include very specific, lengthy explanations that
you will probably discuss in greater detail later in the Discussion. The judgment of what is general
or specific is difficult at first, but with practice and reading
of the scientific literature you will develop e firmer sense
of your audience. In the mouse behavior paper, for example, you
would begin the Introduction at the level of mating behavior
in general, then quickly focus to mouse mating behaviors and
then hormonal regulation of behavior. Lead the reader to your
statement of purpose/hypothesis by focusing your literature review
from the more general context (the big picture e.g., hormonal
modulation of behaviors) to the more specific topic of interest
to you (e.g., role/effects of reproductive hormones, especially
estrogen, in modulating specific sexual behaviors of mice.)
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- What literature should you
look for in your review of what we know about the problem?
Focus your efforts on the primary research journals -
the journals that publish original research articles. Although
you may read some general background references (encyclopedias,
textbooks, lab manuals, style manuals, etc.) to get yourself
acquianted with the subject area, do not cite these, becasue
they contain information that is considered fundamental or "common"
knowledge wqithin the discipline. Cite, instead, articles that
reported specific results relevant to your study. Learn, as soon
as possible, how to find the primary literature (research
journals) and review articles rather than depending on
reference books. The articles listed in the Literature Cited
of relevant papers you find are a good starting point to move
backwards in a line of inquiry. Most academic libraries
support the Citation Index - an index which is useful
for tracking a line of inquiry forward in time. Some of
the newer search engines will actually send you alerts of new
papers that cite particular articles of interest to you. Review
articles are particularly useful because they summarize all
the research done on a narrow subject area over a brief period
of time (a year to a few years in most cases).
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- Be sure to clearly state the
purpose and /or hypothesis that you investigated. When you
are first learning to write in this format it is okay, and actually
preferable, to use a pat statement like, "The purpose of
this study was to...." or "We investigated three possible
mechanisms to explain the ... (1) blah, blah..(2) etc. It is
most usual to place the statement of purpose near the end of
the Introduction, often as the topic sentence of the final paragraph.
It is not necessary (or even desirable) to use the words "hypothesis"
or "null hypothesis", since these are usually implicit
if you clearly state your purpose and expectations.
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- Provide a clear statement
of the rationale for your approach to the problem studied. For
example: State briefly how you approached the problem (e.g.,
you studied oxidative respiration pathways in isolated mitochondria
of cauliflower). This will usually follow your statement of purpose
in the last paragraph of the Introduction. Why did you choose
this kind of experiment or experimental design? What are the
scientific merits of this particular model
system? What advantages does it confer in answering the particular
question(s) you are posing? Do not discuss here the actual techniques
or protocols used in your study (this will be done in
the Materials and Methods); your readers
will be quite familiar with the usual techniques and approaches
used in your field. If you are using a novel (new, revolutionary,
never used before) technique or methodology, the merits of the
new technique/method versus the previously used methods should
be presented in the Introduction.
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MATERIALS AND METHODS
This section is variously called Methods or Methods
and Materials.
1. Function: In this
section you explain clearly how you carried out your study
in the following general structure and organization (details
follow below):
- the subjects used (plant,
animal, human, etc.) and their pre-experiment handling and care,
and when and where the study was carried out (if location and
time are important factors);
- if a field study, a description
of the study site, including the physical and
biological features, and precise location;
- the experimental OR sampling
design (i.e., how the experiment or study was structured.
For example, controls, treatments, the variable(s) measured,
how many samples were collected, replication, etc.);
- the protocol for collecting data,
i.e., how the experimental procedures were carried out, and,
- how the data were analyzed
(statistical procedures used).
Organize your presentation
so your reader will understand the logical flow of the experiment(s);
subheadings work well for this purpose. Each experiment
or procedure should be presented as a unit, even if it was broken
up over time. In general, provide enough quantitative
detail (how much, how long, when, etc.) about your
experimental protocol such that other scientists could reproduce
your experiments. You should also indicate the statistical
procedures used to analyze your results, including the probability
level at which you determined significance (usually at 0.05 probability).
2. Style: The style in this
section should read as if you were verbally describing the conduct
of the experiment. You may use the active voice to a certain
extent, although this section requires more use of third person,
passive constructions than others. Avoid use of the first person
in this section. Remember to use the past tense throughout.
The Methods section is not a step-by-step, directive,
protocol as you might see in your lab manual.
3. Strategy for writing
the Methods section.
4. Methods FAQs.
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Describe the organism(s) used in
the study. This includes giving the source (supplier
or where and how collected), size, how
they were handled before the experiment, what they were fed,
etc. In genetics studies include the strains or genetic stocks
used.
Describe the site where your
field study was conducted. The description must include both
physical and biological characteristics of the
site pertinant to the study aims. Include the date(s) of the
study (e.g., 10-15 April 1994) and the exact location of the
study area. Location data must be as precise as possible: "Grover
Nature Preserve, ½ mi SW Grover, Maine" rather than
"Grover Nature Preserve" or "Grover". When
possible, give the actual latitude and longitude position of
the site (the WWW has sites which provide this service). It is
most often a good idea to include a map (labeled as a
Figure) showing the location in relation to some larger more
recognizable geographic area. Someone else should be able to
go to the exact location of your study if they want to repeat
or check your work, or just visit your study area.
- NOTE: For laboratory studies you
should not report the date and location of the study UNLESS
it is relevant. Most often it is not.
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Describe your experimental
design clearly. Be sure to include the hypotheses
you tested, controls, treatments, variables
measured, how many replicates you had, what you actually
measured, what form the data take, etc. Always
identify treatments by the variable or treatment name, NOT by
an ambiguous, generic name or number (e.g., use "2.5% saline"
rather than "test 1".) When your paper includes more
than one experiment, use subheadings
to help organize your presentation by experiment. A general experimental
design worksheet is available
to help plan your experiments in the core courses.
Describe
the protocol for your study in sufficient detail that other scientists
could repeat your work to verify your findings. Foremost
in your description should be the "quantitative" aspects
of your study - the masses, volumes, incubation times, concentrations,
etc., that another scientist needs in order to duplicate your
experiment. When using standard lab or field methods and instrumentation,
it is not always necessary to explain the procedures (e.g., serial
dilution) or equipment used (e.g., autopipetter) since other
scientists will likely be familiar with them already. You may
want to identify certain types of equipment by brand or category
(e.g., ultracentrifuge vs. prep centrifuge). It is appropriate
to give the source for reagents used parenthetically, e.g., "....poly-l-Lysine (Sigma #1309)."
When using a method described in another published source, you
can save time and words by referring to it and providing the
relevant citation to the
source. Always make sure to describe any modifications you have
made of a standard or published method.
Describe how the data were summarized
and analyzed. Here you will indicate what types of data summaries
and analyses were employed to answer each of the questions or
hypotheses tested.
The information should include:
- how the data were summarized (Means, percent, etc)
and how you are reporting measures of variability (SD,SEM,
etc)
- this lets you avoid having to repeatedly indicate you are
using mean ± SD.
- data transformation (e.g., to normalize or equalize
variances)
- statistical tests used with reference to the particular
questions they address, e.g.,
"A Paired t-test was used to compare
mean flight duration before and after applying stablizers to
the glider's wings."
"One way ANOVA was used to compare
mean weight gain in weight-matched calves fed the three different
rations."
- any other numerical or graphical techniques
used to analyze the data
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Here is some additional advice on particular problems common
to new scientific writers.
Problem: The Methods section is prone to being wordy
or overly detailed.
- Avoid repeatedly using a single sentence to relate a single
action; this results in very lengthy, wordy passages. A related
sequence of actions can be combined into one sentence to improve
clarity and readability:
Problematic Example: This is a very long and wordy
description of a common, simple procedure. It is characterized
by single actions per sentence and lots of unnecessary details.
"The petri dish was placed on
the turntable. The lid was then raised slightly. An inoculating
loop was used to transfer culture to the agar surface. The turntable
was rotated 90 degrees by hand. The loop was moved lightly back
and forth over the agar to spread the culture. The bacteria were
then incubated at 37 C for 24 hr."
Improved Example: Same actions, but all the important
information is given in a single, concise sentence. Note that
superfluous detail and otherwise obvious information has been
deleted while important missing information was added.
"Each plate was placed on a
turntable and streaked at opposing angles with fresh overnight
E. coli culture using an inoculating loop. The bacteria were
then incubated at 37 C for 24 hr."
Best: Here the author assumes the reader has basic
knowledge of microbiological techniques and has deleted other
superfluous information. The two sentences have been combined
because they are related actions.
"Each plate was streaked with
fresh overnight E. coli culture and incubated at 37 C for 24
hr."
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- Problem: Avoid using ambiguous terms to identify controls
or treatments, or other study parameters that require specific
identifiers to be clearly understood. Designators such as Tube
1, Tube 2, or Site 1 and Site 2 are completely meaningless out
of context and difficult to follow in context.
Problematic example: In this example the reader will
have no clue as to what the various tubes represent without having
to constantly refer back to some previous point in the Methods.
"A Spec 20 was used to measure
A600 of Tubes 1,2,
and 3 immediately after chloroplasts
were added (Time 0) and every 2 min. thereafter until the DCIP
was completely reduced. Tube 4's A600
was measured only at Time 0 and
at the end of the experiment."
Improved example: Notice how the substitution
(in red) of treatment and control
identifiers clarifies the passage both in the context of the
paper, and if taken out of context.
"A Spec 20 was used to measure
A600
of the reaction mixtures exposed
to light intensities of 1500, 750, and 350 uE/m2/sec immediately after chloroplasts were added (Time
0) and every 2 min. thereafter until the DCIP was completely
reduced. The A600 of the no light
control was measured only at Time
0 and at the end of the experiment."
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RESULTS
1. Function: The function of the Results section is
to objectively present your key results,
without interpretation, in an orderly and logical
sequence using both illustrative
materials (Tables and Figures) and text.
Summaries of the statistical analyses
may appear either in the text (usually parenthetically) or in
the relevant Tables or Figures (in the legend or as footnotes
to the Table or Figure). The Results section should be organized
around a series of Tables and/or
Figures sequenced to present your key findings in a logical
order. The text of the Results section follows this sequence
and highlights the answers to the questions/hypotheses you investigated.
Important negative results should
be reported, too. Authors usually write the text of the results
section based upon this sequence of Tables and Figures.
2. Style: Write the text of the Results section concisely
and objectively. The passive voice will likely dominate here,
but use the active voice as much as possible. Use the past
tense. Avoid repetitive paragraph structures. Do not interpret
the data here. The transition into interpretive language can
be a slippery slope. Consider the following two examples:
- This example highlights the trend/difference that the author
wants the reader to focus:
The duration of exposure to running
water had a pronounced effect on cumulative seed germination
percentages (Fig. 2). Seeds exposed to the 2-day treatment had
the highest cumulative germination (84%), 1.25 times that of
the 12-h or 5-day groups and 4 times that of controls.
- In contrast, this example strays subtly
into interpretation by referring to optimality (a conceptual
model) and tieing the observed result to that idea:
The results of the germination experiment
(Fig. 2) suggest that the optimal time for running-water treatment
is 2 days. This group showed the highest cumulative germination
(84%), with longer (5 d) or shorter (12 h) exposures producing
smaller gains in germination when compared to the control group.
3. Strategy for Writing
the Results Section
4. Frequently asked questions
(FAQs).
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Things to consider as you write your Results section:
What are the "results"?:
When you pose a testable hypothesis that can be answered experimentally,
or ask a question that can be answered by collecting samples,
you accumulate observations about those organisms or phenomena.
Those observations are then analyzed to yield an answer to the
question. In general, the answer is the " key result".
The above statements apply regardless of the complexity of
the analysis you employ. So, in an introductory course your analysis
may consist of visual inspection of figures and simple calculations
of means and standard deviations; in a later course you may be
expected to apply and interpret a variety of statistical tests.
You instructor will tell you the level of analysis that is expected.
For example, suppose you asked the question, "Is the average height of male students the same
as female students in a pool of Biology majors?" You would first collect height
data from large random samples of male and female students. You
would then calculate the descriptive statistics for those samples
(mean, SD, n, range, etc) and plot these numbers. In a course
where statistical tests are not employed, you would visually
inspect these plots. Suppose you found that male Biology majors
are, on average, 12.5 cm taller than female majors; this is the
answer to the question.
- Notice that the outcome of a statistical analysis is not
a key result, but rather an analytical tool that helps
us understand what is our key result.
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Organize the results section
based on the sequence of Table and Figures you'll include. Prepare
the Tables and Figures as soon
as all the data are analyzed and arrange them in the sequence
that best presents your findings in a logical way. A good strategy
is to note, on a draft of each Table or Figure, the one or two
key results you want to addess in the text portion of the Results.
Simple rules to follow related to Tables and Figures:
- Tables and Figures are assigned
numbers separately and in the sequence that you will refer
to them from the text.
- The first Table you refer to is Table 1, the next Table 2
and so forth.
- Similarly, the first Figure is Figure 1, the next Figure
2, etc.
- Each Table or Figure must include a brief description
of the results being presented and other necessary information
in a legend.
- Table legends go above the Table; tables are read
from top to bottom.
- Figure legends go below the figure; figures are usually
viewed from bottom to top.
- When referring to
a Figure from the text, "Figure" is abbreviated
as Fig.,e.g.,
Fig. 1. Table is never abbreviated, e.g., Table 1.
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The body of the Results section is a
text-based presentation of the key findings which includes references
to each of the Tables and Figures. The text should guide
the reader through your results stressing the key results which
provide the answers to the question(s) investigated. A major
function of the text is to provide clarifying information. You
must refer to each Table and/or Figure individually and in sequence
(see numbering sequence),
and clearly indicate for the reader the key results that each
conveys. Key results depend on your questions, they might include
obvious trends, important differences, similarities, correlations,
maximums, minimums, etc.
Some things to avoid:
- Do not reiterate each value from a Figure or Table
- only the key result or trends that each conveys.
- Do not present the same data in both a Table and Figure
- this is considered redundant and a waste of space and energy.
Decide which format best shows the result and go with it.
- Do not report raw data values when they can be summarized
as means, percents, etc.
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Statistical test summaries (test
name, p-value) are usually reported parenthetically in
conjunction with the biological results they support. Always
report your results with parenthetical reference to the statistical
conclusion that supports your finding (if statistical tests are
being used in your course). This parenthetical reference should
include the statistical test used and the level of significance
(test statistic and DF are optional). For example, if you found
that the mean height of male Biology majors was significantly
larger than that of female Biology majors, you might report this
result (in blue) and your statistical conclusion (shown in red)
as follows:
"Males (180.5 ± 5.1 cm;
n=34) averaged 12.5 cm taller than females (168 ± 7.6
cm; n=34) in the AY 1995 pool of Biology majors (two-sample t-test, t = 5.78, 33 d.f., p <
0.001)."
Note that the report of the key result
(shown in blue) would be identical in a paper written for a course
in which statistical testing is not employed - the section shown
in red would simply not appear.
- Avoid devoting whole sentences to report a statistical outcome
alone.
- Two notes about the use of the word significant(ly).
- In scientific studies, the use of this word implies that
a statistical test was employed to make a decision about the
data; in this case the test indicated a larger difference in
mean heights than you would expect to get by chance alone. Limit
the use of the word "significant" to this purpose only.
- If your parenthetical statistical information includes a
p-value that is significant, it is unncecssary (and redundant)
to use the word "significant" in the body of the sentence
(see example above).
Present the results of your experiment(s)
in a sequence that will logically support (or provide evidence
against) the hypothesis, or answer the question, stated in the
Introduction. For example, in reporting a study of the effect
of an experimental diet on the skeletal mass of the rat, consider
first giving the data on skeletal mass for the rats fed the control
diet and then give the data for the rats fed the experimental
diet.
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Report negative results
- they are important! If you did not get the anticipated
results, it may mean your hypothesis was incorrect and needs
to be reformulated, or perhaps you have stumbled onto something
unexpected that warrants further study. In either case, your
results may be of importance to others even though they did not
support your hypothesis. Do not fall into the trap of thinking
that results contrary to what you expected are necessarily "bad
data". If you carried out the work well, they are simply
your results and need interpretation. Many important discoveries
can be traced to "bad data".
Always enter the appropriate units
when reporting data or summary statistics.
- for an individual value you would write, "the mean length was 10 m", or, "the maximum time was 140 min."
- When including a measure of variability, place the unit after
the error value, e.g., "...was 10
± 2.3 m".
- Likewise place the unit after the last in a series
of numbers all having the same unit. For example: "lengths of 5, 10, 15, and 20 m",
or "no differences were observed after
2, 4, 6, or 8 min. of incubation".
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Discussion
| strategy
| FAQs | style
| approach | use
of literature | results in discussion
|
1. Function: The function of the Discussion is to interpret
your results in light of what was already
known about the subject of the investigation, and to explain
our new understanding of the problem after taking your results
into consideration. The Discussion will always connect to the
Introduction by way of the question(s)
or hypotheses you posed and the literature you cited, but it
does not simply repeat or rearrange the Introduction. Instead,
it tells how your study has moved us forward from the place you
left us at the end of the Introduction.
Fundamental questions to answer here include:
- Do your results provide answers to your testable hypotheses?
If so, how do you interpret your findings?
- Do your findings agree with what others have shown? If not,
do they suggest an alternative explanation or perhaps a unforseen
design flaw in your experiment (or theirs?)
- Given your conclusions, what is our new understanding of
the problem you investigated and outlined in the Introduction?
- If warranted, what would be the next step in your study,
e.g., what experiments would you do next?
2. Style: Use the active
voice whenever possible in this section. Watch out for wordy
phrases; be concise and make your points clearly. Use of the
first person is okay, but too much use of the first person may
actually distract the reader from the main points.
3. Approach: Organize
the Discussion to address each of the experiments or studies
for which you presented results; discuss each in the same sequence
as presented in the Results, providing your interpretation of
what they mean in the larger context of the problem. Do not waste
entire sentences restating your results; if you need to remind
the reader of the result to be discussed, use "bridge sentences"
that relate the result to the interpretation:
"The slow response of the lead-exposed
neurons relative to controls suggests that...[interpretation]".
You will necessarily make reference
to the findings of others in order to support your interpretations.Use
subheadings, if need be, to help organize
your presentation. Be wary of mistaking the reiteration of a
result for an interpretation, and make sure that no
new results are presented here that rightly belong in the
results.
You must relate your work
to the findings of other studies - including previous studies
you may have done and those of other investigators. As stated
previously, you may find crucial information in someone else's
study that helps you interpret your own data, or perhaps you
will be able to reinterpret others' findings in light of yours.
In either case you should discuss reasons for similarities and
differences between yours and others' findings. Consider how
the results of other studies may be combined with yours to derive
a new or perhaps better substantiated understanding of the problem.
Be sure to state the conclusions that can be drawn from your
results in light of these considerations. You may also choose
to briefly mention further studies you would do to clarify your
working hypotheses. Make sure to reference
any outside sources as shown in the Introduction section.
Do not introduce new results in
the Discussion. Although you might occasionally include in
this section tables and figures which help explain something
you are discussing, they must not contain new data (from your
study) that should have been presented earlier. They might be
flow diagrams, accumulation of data from the literature, or something
that shows how one type of data leads to or correlates with another,
etc. For example, if you were studying a membrane-bound transport
channel and you discovered a new bit of information about its
mechanism, you might present a diagram showing how your findings
helps to explain the channel's mechanism.
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Acknowledgments (included as
needed)
| FAQs
|
If, in your experiment, you received any significant help
in thinking up, designing, or carrying out the work, or received
materials from someone who did you a favor by supplying them,
you must acknowledge their assistance and the service or material
provided. Authors always acknowledge outside reviewers
of their drafts (in PI courses, this would be done only
if an instructor or other individual critiqued the draft prior
to evaluation) and any sources of funding that supported
the research. Although usual style requirements (e.g., 1st person,
objectivity) are relaxed somewhat here, Acknowledgments are always
brief and never flowery.
- Place the Acknowledgments between
the Discussion and the Literature Cited.
Literature Cited
1. Function: The Literature Cited section gives an
alphabetical listing (by first author's last name) of the references
that you actually cited in the body of your paper. Instructions
for writing full citations for various sources are given
in on separate page. A complete format list for virtually all
types of publication may be found in Huth
and others(1994).
NOTE: Do not label this section "Bibliography".
A bibliography contains references that you may have read but
have not specifically cited in the text. Bibliography sections
are found in books and other literary writing, but not scientific
journal-style papers.
2. Format and Instructions
for standard full citations of sources.
3. Literature Cited FAQs.
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Appendices
| FAQs | Function | Headings
| Types of Content | Tables
and Figures
Function: An
Appendix contains information that is non-essential to understanding
of the paper, but may present information that further clarifies
a point without burdening the body of the presentation. An appendix
is an optional part of the paper, and is only rarely found
in published papers.
Headings: Each Appendix
should be identified by a Roman numeral in sequence, e.g., Appendix
I, Appendix II, etc. Each appendix should contain different material.
Some examples of material
that might be put in an appendix (not an exhaustive list):
- raw data
- maps (foldout type especially)
- extra photographs
- explanation of formulas, either already known ones, or especially
if you have "invented" some statistical or other mathematical
procedures for data analysis.
- specialized computer programs for a particular procedure
- full generic names of chemicals or compounds that you have
referred to in somewhat abbreviated fashion or by some common
name in the text of your paper.
- diagrams of specialized apparati.
Figures and Tables in Appendices
Figures and Tables are often found in an appendix. These should
be formatted as discussed previously (see Tables
and Figures), but are numbered in a separate sequence from
those found in the body of the paper. So, the first Figure in
the appendix would be Figure 1, the first Table would be Table
1, and so forth. In situations when multiple appendices are used,
the Table and Figure numbering must indicate the appendix number
as well (see Huth and others, 1994).